Biological Properties oF Cnicus benedictus Using Zinc Nanoparticles (ZnNPs).

Abstract

Cnicus benedictus, often known as Blessed Thistle, was synthesised using zinc nanoparticles (ZnNPs) in this work to learn more about its biological characteristics and its uses in nanomedicine and natural medicine.  Analgesic, antibacterial, and anticancer effects are the traditional uses of the medicinal plant cnicus benedictus.  This work includes the biosynthesis of zinc nanoparticles using water-based Cnicus benedictus extracts. The nanoparticles were then characterised by using UV-Vis spectroscopy, FTIR, XRD, and SEM to validate their creation, size, shape, and the functional groups responsible for stabilising them.  A battery of tests were run on the biologically produced ZnNPs to determine their cytotoxic, antibacterial, and antioxidant capabilities. Results showed strong antioxidant capacity as shown by DPPH and ABTS tests, as well as antimicrobial efficacy against a wide variety of bacteria, including those with Gram-positive and Gram-negative characteristics. Additionally, several cancer cell lines showed encouraging anti-proliferative properties in vitro cytotoxic study.  Combining the advantages of plant-derived phytochemicals with the improved bioavailability and functionality of zinc-based nanoparticles, the results show that Cnicus benedictus-mediated ZnNPs have great potential as bioactive agents for pharmaceutical and biomedical uses.  To further understand the in vivo effectiveness and safety of the mechanisms of action, more research is needed.